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Benoit Coulombe, PhD

Director, Department of Translational Proteomics, IRCM

Bell-Bombardier Research Chair, IRCM

Professor, Department of Biochemistry & Molecular Medicine, U of Montréal

Leader of the 37TrillionCells initiative

Keywords: Diabetes, single-cell (SC) proteomics, mass spectrometry (MS), phage display, recombinant antibodies, proteogenomics, cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), pancreatic cells, peripheral blood mononuclear cells (PBMCs), adipose tissue, cell surface proteins, cell-based interceptive medicine, predictive medicine

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Towards a cell-based understanding of diabetes

Our lab is mapping the phenotypic trajectory of many thousands of individual patient cells at various times in the progression of diabetes, seeking to uncover totally ignored cell functions and novel cell surface effectors that regulate key signaling events and cell-cell interactions.

Cell-based interceptive medicine (Médecine cellulaire d'interception) is a new medical paradigm that aims to treat diseases at the first signs of disruption of gene expression programs in cells that branch off from health to disease. We trust that one of the most powerful approach to uncover modulations of gene expression programs in individual cells is based on single-cell (SC) proteome profiling, as this method directly quantifies the functional actors of the cell (the proteins) as well as their regulatory posttranslational modifications. The cell being the basic functional unit of a human being, focusing on SC proteomics promises to revolutionize our understanding of many diseases, including diabetes which affects more than 500M people worldwide and provokes severe complications. In collaboration with the IRCM medical clinic to implement cell-based interceptive medicine in Qc/Canada, our laboratory is developing methods for epitope-based and mass spectrometry-based SC proteomics applied to the onset and progression of diabetes in patients and various human and animal models.

We are part of the 37TrillionCells Initiative

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a research partner of the EU LifeTime initiative
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Our group adopts a resolutely innovative modus operandi, which contributes to shaking up our classical methods by creating a transdisciplinary and open ecosystem where totally distinct approaches collide to allow both the study of the atomic structure of proteins and their organization in complex networks, both the analysis of microscopic model systems and of patient cohorts, both the screening of small molecules and their development to create affordable drugs.

To join the Coulombe lab as a graduate student or a postdoctoral fellow, please send a motivation letter and a complete CV by email directly to Benoit.Coulombe@ircm.qc.ca. Only candidates of interest will be contacted.

RECENT PUBLICATIONS (2018-21)

Interactome Analysis of KIN (Kin17) Shows New Functions of This Protein

Gaspar VP, Ramos AC, Philippe Cloutier, Pattaro Júnior JR, Duarte Junior FF, Bouchard A, Seixas FAV, Coulombe B, Fernandez MA

Curr. Issues Mol. Biol. 2021 Jul 22; 43(2), 767-781.

Discovery of antivirals using phage display

Sokullu E, Gauthier MS, Coulombe B

Viruses. 2021 Jun 10;13(6):1120.

Recurrent chromosomal translocations in sarcomas create a mega-complex that mislocalizes NuA4/TIP60 to Polycomb target loci

Sudarshan D, Avvakumov N, Lalonde ME, Alerasool N, Jacquet K, Mameri A, Rousseau J, Lambert JP, Paquet E, Thonta Setty S, Loehr J, Gingras AC, Coulombe B, Taipale M, Doyon Y, Cote J

https://doi.org/10.1101/2021.03.26.436670

Biallelic pathogenic variants in LSM7impair assembly of LSM complexes, impairs neurodevelopment in zebrafish and may be associated with an ultra rare neurodevelopmental and neurodegenerative disorder

Derksen A, Shih HY, Forget D, Darbelli L, Tran LT, Poitras C, Guerrero K, Tharun S, Alkuraya FS, Kurdi WI, Nguyen CTE, Laberge AM, Si Y, Gauthier MS, Bonkowsky J*, Coulombe B*, Bernard G*. (*Co-senior authors)

HGG Adv. 2021. In Press.

POLR3-related leukodystrophy: How do mutations affecting RNA polymerase III subunits cause hypomyelination?

Coulombe B, Derksen A, La Piana R, Brais B, Gauthier MS, Bernard G.

Fac Rev. 2021 Feb 5;10:12.

De novo variants in POLR3B cause ataxia, spasticity, and demyelinating neuropathy

Djordjevic D, Pinard M, Gauthier MS, Smith-Hicks C, Hoffman TL, Wolf NI, Oegema R, van Binsbergen E, Baskin B, Bernard G, Fribourg S, Coulombe B, Yoon G.

Am J Hum Genet. 2021 Jan 7;108(1):186-193.

Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the PAQosome

Cloutier P, Poitras C, Faubert D, Bouchard A, Blanchette M, Gauthier MS, Coulombe B.

J Proteome Res. 2020 Jan 3;19(1):18-27.

Ser-Phosphorylation of PCSK9 (Proprotein Convertase Subtilisin-Kexin 9) by Fam20C (Family With Sequence Similarity 20, Member C) Kinase Enhances Its Ability to Degrade the LDLR (Low-Density Lipoprotein Receptor)

Ben Djoudi Ouadda A, Gauthier MS, Susan-Resiga D, Girard E, Essalmani R, Black M, Marcinkiewicz J, Forget D, Hamelin J, Evagelidis A, Ly K, Day R, Galarneau L, Corbin F, Coulombe B, Çaku A, Tagliabracci VS, Seidah NG.

Arterioscler Thromb Vasc Biol. 2019 Oct;39(10):1996-2013.

The leukodystrophy mutation Polr3b R103H causes homozygote mouse embryonic lethality and impairs RNA polymerase III biogenesis

Choquet K, Pinard M, Yang S, Moir RD, Poitras C, Dicaire MJ, Sgarioto N, Larivière R, Kleinman CL, Willis IM, Gauthier MS, Coulombe B, Brais B.

Mol Brain. 2019 Jun 20;12(1):59.

Leukodystrophy-associated POLR3A mutations down-regulate the RNA polymerase III transcript and important regulatory RNA BC200

Choquet K, Forget D, Meloche E, Dicaire MJ, Bernard G, Vanderver A, Schiffmann R, Fabian MR, Teichmann M, Coulombe B, Brais B, Kleinman CL.

J Biol Chem. 2019 May 3;294(18):7445-7459.

Role of the PAQosome in Regulating Arrangement of Protein Quaternary Structure in Health and Disease

Gauthier MS, Cloutier P, Coulombe B.

Adv Exp Med Biol. 2018;1106:25-36.

How do our cells build their protein interactome?

Coulombe B, Cloutier P, Gauthier MS.

Nat Commun. 2018 Jul 27;9(1):2955.

Posttranslational modification of proprotein convertase subtilisin/kexin type 9 is differentially regulated in response to distinct cardiometabolic treatments as revealed by targeted proteomics

Gauthier MS, Awan Z, Bouchard A, Champagne J, Tessier S, Faubert D, Chabot K, Garneau PY, Rabasa-Lhoret R, Seidah NG, Ridker PM, Genest J, Coulombe B.

J Clin Lipidol. 2018 Jul-Aug;12(4):1027-1038.

Bi-allelic Mutations in EPRS, Encoding the Glutamyl-Prolyl-Aminoacyl-tRNA Synthetase, Cause a Hypomyelinating Leukodystrophy

Mendes MI, Gutierrez Salazar M, Guerrero K, Thiffault I, Salomons GS, Gauquelin L, Tran LT, Forget D, Gauthier MS, Waisfisz Q, Smith DEC, Simons C, van der Knaap MS, Marquardt I, Lemes A, Mierzewska H, Weschke B, Koehler W, Coulombe B, Wolf NI, Bernard G.

Am J Hum Genet. 2018 Apr 5;102(4):676-684.

The PAQosome, an R2TP-Based Chaperone for Quaternary Structure Formation

Houry WA, Bertrand E, Coulombe B.

Trends Biochem Sci. 2018 Jan;43(1):4-9.

 
 

TEAM

Dr. Benoit Coulombe
Dr. Benoit Coulombe
Director
Dr. Marie-Soleil Gauthier
Dr. Marie-Soleil Gauthier
Senior Associate Researcher
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Dr. Maxime Pinard
Associate Researcher
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Dr. Esen Sokullu
Postdoctoral Fellow
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Asmae Moursli
PhD student
Christian Poitras
Christian Poitras
Systems Analyst
Golden Marble
Vijaya Madhoo
Administrative Assistant
Diane Forget
Diane Forget
Honorary Lab Member

2020-06-26 :

Diane Forget prend sa retraite après 27 ans de service dévoué pour le laboratoire. Elle a eu une contribution exceptionnelle pour nos publications, la formation de personnel, l'administration des fonds de recherche et la planification des espaces de laboratoire. Avec toute notre reconnaissance, merci et bonne continuation.

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