top of page

Exploring the Universe of Protein-Protein Interactions

Mission
Protein folding figure
Mission

Discovery of the PAQosome

PaqosomeNetworkAndComplex.png

Our Technology Platforms

  • Affinity Purification coupled with Mass Spectrometry (AP-MS)
    Purification and characterization of soluble protein complexes

  • Proximity-dependent labeling coupled with Mass spectrometry (BioID-MS)
    Characterization of insoluble protein complexes

  • Mass Spectrometry Immuno Affinity (MSIA)
    Targeted protein quantification

  • FAB-Phage Display screening (PhD)
    Unbiased discovery of FAB-phages recognizing specific cell epitopes

  • Quantitative Single-Cell Proteomics (SCP)
    Identification of proteins at single-cell resolution

  • Small-molecule screening (SMS)
    Discovery of small molecules affecting biological/biochemical functions

  • Artificial Intelligence-Alphafold2 multimer-Binary (AI-AF2m-Binary)
    Determination of pairwise protein-protein interactions in the AlphaFold database

MOST RECENT ARTICLE

Discovery of a drug that stimulates protein complex assembly

Pinard M, Dastpeyman S, Poitras C, Bernard G, Gauthier MS, Coulombe B.

Riluzole partially restores RNA polymerase III complex assembly in cells expressing the leukodystrophy-causative variant POLR3B R103H

Mol Brain. 2022 Nov 30;15(1):98. doi: 10.1186/s13041-022-00974-z.

Schematic representation of Pol III assembly defects caused by various mutated subunits -

Schematic representation of Pol III assembly defects caused by various mutated subunits

Recent Article

FEATURED ARTICLES

PAQosome Subunit RPAP3 Binds Ribosomal Preassembly Complexes

PAQosome Subunit RPAP3 Binds Ribosomal Preassembly Complexes

Discovery of a novel neurodegenerative disorder and causative mutations

Djordjevic D, Pinard M, Gauthier MS, Smith-Hicks C, Hoffman TL, Wolf NI, Oegema R, van Binsbergen E, Baskin B, Bernard G, Fribourg S, Coulombe B, Yoon G.

De novo variants in POLR3B cause ataxia, spasticity, and demyelinating neuropathy

Am J Hum Genet. 2021 Jan 7;108(1):186-193. doi: 10.1016/j.ajhg.2020.12.002

Model of POLR3 in transcription initiation state

Model of POLR3 in transcription initiation state

Representation of putative alternative PAQosomes

Representation of putative alternative PAQosomes

Schematic of the PAQosome Structure, Adaptors, and Clients

Schematic of the PAQosome Structure, Adaptors, and Clients

Discovery of leukodystrophy-causing mutations that target assembly of RNA pol III

Thiffault I, Wolf NI, Forget D, Guerrero K, Tran LT, Choquet K, Lavallée-Adam M, Poitras C, Brais B, Yoon G, Sztriha L, Webster RI, Timmann D, van de Warrenburg BP, Seeger J, Zimmermann A, Máté A, Goizet C, Fung E, van der Knaap MS, Fribourg S, Vanderver A, Simons C, Taft RJ, Yates JR 3rd, Coulombe B, Bernard G.

Recessive mutations in POLR1C cause a leukodystrophy by impairing biogenesis of RNA polymerase III

Nat Commun. 2015 Jul 7;6:7623. doi: 10.1038/ncomms8623.

Impact of POLR1C mutations on polymerase assembly and nuclear import

Impact of POLR1C mutations on polymerase assembly and nuclear import

R2TP substrates

R2TP substrates

Selected publications

Using machine learning to build protein interaction networks

Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Thérien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B.

Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme

Mol Cell. 2007 Jul 20;27(2):262-274. doi: 10.1016/j.molcel.2007.06.027.

Application of machine learning to construct an interaction network for the human RNA polymerase II transcription machinery, leading to discovery of the 7SK capping enzyme

RNAP II Transcription Machinery

A Network of Protein Complexes Involving the RNAP II Basal Transcription Machinery

TEAM

benoit_coulombe_square.png

Dr. Benoit Coulombe

Director

Biography

esen_sokullu.png

Dr. Esen Sokullu

Post Doctoral Fellow

Christian Poitras.jpg

Christian Poitras

System Analyst

maxime_pinard_small.png

Dr. Maxime Pinard

Senior Associate Researcher

Asmae_Moursli_small.png

Asmae Moursli

PhD Student

Golden Marble

Vijaya Madoo

Administrative Assistant

Team

L’Institut de recherches cliniques de Montréal n’est pas responsable du contenu de ce site Web.

The Montreal Clinical Research Institute is not responsible for the content of this website.

bottom of page