Transcribing RNA polymerase

Benoit Coulombe, PhD

Director, Department of Translational Proteomics, IRCM

Bell-Bombardier Research Chair, IRCM

Professor, Department of Biochemistry & Molecular Medicine, U of Montréal

Leader of the 37TrillionCells initiative

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Our lab is developing single-cell proteogenomics to better comprehend, diagnose and treat diabetes, in collaboration with the IRCM clinic

Cell-based interceptive medicine is a new medical paradigm that aims to treat diseases at the first signs of disruption of gene expression programs in cells that branch off from health to disease. Longitudinal characterization of gene expression networks in individual cells by microfluidic multi-omics (genomic, transcriptomic and proteomic analyzes at the single-cell level) makes it possible to detect these bifurcations, track cell phenotypes, and ultimately develop treatments to intercept them before tissue damage and symptoms present themselves. We mainly apply this strategy to diabetes and its complications.

We are part of the 37TrillionCells Initiative

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a research partner of the EU LifeTime initiative
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Our group adopts a resolutely innovative modus operandi, which contributes to shaking up our classical methods by creating a transdisciplinary and open ecosystem where totally distinct approaches collide to allow both the study of the atomic structure of proteins and their organization in complex networks, both the analysis of microscopic model systems and of patient cohorts, both the screening of small molecules and their development to create affordable drugs.

To join the Coulombe lab as a graduate student or a postdoctoral fellow, please send a motivation letter and a complete CV by email directly to Benoit.Coulombe@ircm.qc.ca. Only candidates of interest will be contacted.

RECENT PUBLICATIONS (2018-21)

Discovery of antivirals using phage display

Sokullu E, Gauthier MS, Coulombe B

Bacterial Viruses. 2021, In Press

Recurrent chromosomal translocations in sarcomas create a mega-complex that mislocalizes NuA4/TIP60 to Polycomb target loci

Sudarshan D, Avvakumov N, Lalonde ME, Alerasool N, Jacquet K, Mameri A, Rousseau J, Lambert JP, Paquet E, Thonta Setty S, Loehr J, Gingras AC, Coulombe B, Taipale M, Doyon Y, Cote J

https://doi.org/10.1101/2021.03.26.436670

Biallelic pathogenic variants in LSM7impair assembly of LSM complexes, impairs neurodevelopment in zebrafish and may be associated with an ultra rare neurodevelopmental and neurodegenerative disorder

Derksen A, Shih HY, Forget D, Darbelli L, Tran LT, Poitras C, Guerrero K, Tharun S, Alkuraya FS, Kurdi WI, Nguyen CTE, Laberge AM, Si Y, Gauthier MS, Bonkowsky J*, Coulombe B*, Bernard G*. (*Co-senior authors)

HGG Adv. 2021. In Press.

POLR3-related leukodystrophy: How do mutations affecting RNA polymerase III subunits cause hypomyelination?

Coulombe B, Derksen A, La Piana R, Brais B, Gauthier MS, Bernard G.

Fac Rev. 2021 Feb 5;10:12.

De novo variants in POLR3B cause ataxia, spasticity, and demyelinating neuropathy

Djordjevic D, Pinard M, Gauthier MS, Smith-Hicks C, Hoffman TL, Wolf NI, Oegema R, van Binsbergen E, Baskin B, Bernard G, Fribourg S, Coulombe B, Yoon G.

Am J Hum Genet. 2021 Jan 7;108(1):186-193.

Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the PAQosome

Cloutier P, Poitras C, Faubert D, Bouchard A, Blanchette M, Gauthier MS, Coulombe B.

J Proteome Res. 2020 Jan 3;19(1):18-27.

Ser-Phosphorylation of PCSK9 (Proprotein Convertase Subtilisin-Kexin 9) by Fam20C (Family With Sequence Similarity 20, Member C) Kinase Enhances Its Ability to Degrade the LDLR (Low-Density Lipoprotein Receptor)

Ben Djoudi Ouadda A, Gauthier MS, Susan-Resiga D, Girard E, Essalmani R, Black M, Marcinkiewicz J, Forget D, Hamelin J, Evagelidis A, Ly K, Day R, Galarneau L, Corbin F, Coulombe B, Çaku A, Tagliabracci VS, Seidah NG.

Arterioscler Thromb Vasc Biol. 2019 Oct;39(10):1996-2013.

The leukodystrophy mutation Polr3b R103H causes homozygote mouse embryonic lethality and impairs RNA polymerase III biogenesis

Choquet K, Pinard M, Yang S, Moir RD, Poitras C, Dicaire MJ, Sgarioto N, Larivière R, Kleinman CL, Willis IM, Gauthier MS, Coulombe B, Brais B.

Mol Brain. 2019 Jun 20;12(1):59.

Leukodystrophy-associated POLR3A mutations down-regulate the RNA polymerase III transcript and important regulatory RNA BC200

Choquet K, Forget D, Meloche E, Dicaire MJ, Bernard G, Vanderver A, Schiffmann R, Fabian MR, Teichmann M, Coulombe B, Brais B, Kleinman CL.

J Biol Chem. 2019 May 3;294(18):7445-7459.

Role of the PAQosome in Regulating Arrangement of Protein Quaternary Structure in Health and Disease

Gauthier MS, Cloutier P, Coulombe B.

Adv Exp Med Biol. 2018;1106:25-36.

How do our cells build their protein interactome?

Coulombe B, Cloutier P, Gauthier MS.

Nat Commun. 2018 Jul 27;9(1):2955.

Posttranslational modification of proprotein convertase subtilisin/kexin type 9 is differentially regulated in response to distinct cardiometabolic treatments as revealed by targeted proteomics

Gauthier MS, Awan Z, Bouchard A, Champagne J, Tessier S, Faubert D, Chabot K, Garneau PY, Rabasa-Lhoret R, Seidah NG, Ridker PM, Genest J, Coulombe B.

J Clin Lipidol. 2018 Jul-Aug;12(4):1027-1038.

Bi-allelic Mutations in EPRS, Encoding the Glutamyl-Prolyl-Aminoacyl-tRNA Synthetase, Cause a Hypomyelinating Leukodystrophy

Mendes MI, Gutierrez Salazar M, Guerrero K, Thiffault I, Salomons GS, Gauquelin L, Tran LT, Forget D, Gauthier MS, Waisfisz Q, Smith DEC, Simons C, van der Knaap MS, Marquardt I, Lemes A, Mierzewska H, Weschke B, Koehler W, Coulombe B, Wolf NI, Bernard G.

Am J Hum Genet. 2018 Apr 5;102(4):676-684.

The PAQosome, an R2TP-Based Chaperone for Quaternary Structure Formation

Houry WA, Bertrand E, Coulombe B.

Trends Biochem Sci. 2018 Jan;43(1):4-9.

 
 

TEAM

Dr. Benoit Coulombe
Dr. Benoit Coulombe
Director
Dr. Marie-Soleil Gauthier
Dr. Marie-Soleil Gauthier
Senior Associate Researcher
maxime_pinard_small.png
Dr. Maxime Pinard
Associate Researcher
esen_sokullu.png
Dr. Esen Sokullu
Postdoctoral Fellow
Asmae_Moursli_small.png
Asmae Moursli
PhD student
Christian Poitras
Christian Poitras
Systems Analyst
Golden Marble
Vijaya Madhoo
Administrative Assistant
Diane Forget
Diane Forget
Honorary Lab Member

2020-06-26 :

Diane Forget prend sa retraite après 27 ans de service dévoué pour le laboratoire. Elle a eu une contribution exceptionnelle pour nos publications, la formation de personnel, l'administration des fonds de recherche et la planification des espaces de laboratoire. Avec toute notre reconnaissance, merci et bonne continuation.

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