Benoit Coulombe lab

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Our Goal

To discover, characterize and correct disruptions in the structure of protein interaction networks that cause diseases, with the vision of advancing biology and discovering new drugs.

 

Call for papers

Integrative Omics at Single-Cell Resolution

Science Direct - Methods

Our Technology Platforms

  • Affinity Purification coupled with Mass Spectrometry (AP-MS)
    Purification and characterization of soluble protein complexes

  • Proximity-dependent labeling coupled with Mass spectrometry (BioID-MS)
    Characterization of insoluble protein complexes

  • Mass Spectrometry Immuno Affinity (MSIA)
    Targeted protein quantification

  • FAB-Phage Display screening (PhD)
    Unbiased discovery of FAB-phages recognizing specific cell epitopes

  • Quantitative Single-Cell Proteomics (SCP)
    Identification of proteins at single-cell resolution

  • Small-molecule screening (SMS)
    Discovery of small molecules affecting biological/biochemical functions

MOST RECENT ARTICLE

Discovery of a drug that stimulates protein complex assembly

Pinard M, Dastpeyman S, Poitras C, Bernard G, Gauthier MS, Coulombe B.

Riluzole partially restores RNA polymerase III complex assembly in cells expressing the leukodystrophy-causative variant POLR3B R103H

Mol Brain. 2022 Nov 30;15(1):98. doi: 10.1186/s13041-022-00974-z.

Schematic representation of Pol III assembly defects caused by various mutated subunits -

Schematic representation of Pol III assembly defects caused by various mutated subunits

 

FEATURED ARTICLES

PAQosome Subunit RPAP3 Binds Ribosomal Preassembly Complexes

PAQosome Subunit RPAP3 Binds Ribosomal Preassembly Complexes

Discovery of a novel neurodegenerative disorder and causative mutations

Djordjevic D, Pinard M, Gauthier MS, Smith-Hicks C, Hoffman TL, Wolf NI, Oegema R, van Binsbergen E, Baskin B, Bernard G, Fribourg S, Coulombe B, Yoon G.

De novo variants in POLR3B cause ataxia, spasticity, and demyelinating neuropathy

Am J Hum Genet. 2021 Jan 7;108(1):186-193. doi: 10.1016/j.ajhg.2020.12.002

Model of POLR3 in transcription initiation state

Model of POLR3 in transcription initiation state

Representation of putative alternative PAQosomes

Representation of putative alternative PAQosomes

Schematic of the PAQosome Structure, Adaptors, and Clients

Schematic of the PAQosome Structure, Adaptors, and Clients

Discovery of leukodystrophy-causing mutations that target assembly of RNA pol III

Thiffault I, Wolf NI, Forget D, Guerrero K, Tran LT, Choquet K, Lavallée-Adam M, Poitras C, Brais B, Yoon G, Sztriha L, Webster RI, Timmann D, van de Warrenburg BP, Seeger J, Zimmermann A, Máté A, Goizet C, Fung E, van der Knaap MS, Fribourg S, Vanderver A, Simons C, Taft RJ, Yates JR 3rd, Coulombe B, Bernard G.

Recessive mutations in POLR1C cause a leukodystrophy by impairing biogenesis of RNA polymerase III

Nat Commun. 2015 Jul 7;6:7623. doi: 10.1038/ncomms8623.

Impact of POLR1C mutations on polymerase assembly and nuclear import

Impact of POLR1C mutations on polymerase assembly and nuclear import

R2TP substrates

R2TP substrates

 
 

TEAM

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Dr. Benoit Coulombe

Director

Biography

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Dr. Esen Sokullu

Post Doctoral Fellow

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Christian Poitras

System Analyst

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Dr. Maxime Pinard

Senior Associate Researcher

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Asmae Moursli

PhD Student

Golden Marble

Vijaya Madoo

Administrative Assistant

 
 

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