Benoit Coulombe lab

Translational Proteomics

News From our Lab

Biography

Our Goal

To discover, characterize and correct disruptions in the structure of protein interaction networks that cause diseases, with the vision of advancing biology and discovering new drugs.

Call for papers

Integrative Omics at Single-Cell Resolution

See https://www.sciencedirect.com/journal/methods/about/call-for-papers

Our Technology Platforms

Affinity Purification coupled with Mass Spectrometry (AP-MS)
Purification and characterization of soluble protein complexes

Proximity-dependent labeling coupled with Mass spectrometry (BioID-MS)
Characterization of insoluble protein complexes

Mass Spectrometry Immuno Affinity (MSIA)
Targeted protein quantification

FAB-Phage Display screening (PhD)
Unbiased discovery of FAB-phages recognizing specific cell epitopes

Quantitative Single-Cell Proteomics (SCP)
Identification of proteins at single-cell resolution

Small-molecule screening (SMS)
Discovery of small molecules affecting biological/biochemical functions

MOST RECENT ARTICLE

Discovery of a drug that stimulates protein complex assembly

Pinard M, Dastpeyman S, Poitras C, Bernard G, Gauthier MS, Coulombe B.

Riluzole partially restores RNA polymerase III complex assembly in cells expressing the leukodystrophy-causative variant POLR3B R103H

Mol Brain. 2022 Nov 30;15(1):98. doi: 10.1186/s13041-022-00974-z.

Schematic representation of Pol III assembly defects caused by various mutated subunits

FEATURED ARTICLES

Discovery of a novel mode of substrate selection by the PAQosome

Pinard M, Cloutier P, Poitras C, Gauthier MS, Coulombe B.

Unphosphorylated Form of the PAQosome Core Subunit RPAP3 Binds Ribosomal Preassembly Complexes to Modulate Ribosome Biogenesis

J Proteome Res. 2022 Apr 1;21(4):1073-1082. doi: 10.1021/acs.jproteome.1c00938

PAQosome Subunit RPAP3 Binds Ribosomal Preassembly Complexes

Discovery of a novel neurodegenerative disorder and causative mutations

Djordjevic D, Pinard M, Gauthier MS, Smith-Hicks C, Hoffman TL, Wolf NI, Oegema R, van Binsbergen E, Baskin B, Bernard G, Fribourg S, Coulombe B, Yoon G.

De novo variants in POLR3B cause ataxia, spasticity, and demyelinating neuropathy

Am J Hum Genet. 2021 Jan 7;108(1):186-193. doi: 10.1016/j.ajhg.2020.12.002

Model of POLR3 in transcription initiation state

A mechanistic model for interactome biogenesis

Coulombe B, Cloutier P, Gauthier MS.

How do our cells build their protein interactome?

Nat Commun. 2018 Jul 27;9(1):2955. doi: 10.1038/s41467-018-05448-2.

Representation of putative alternative PAQosomes

Summary of the PAQosome structure and function

Houry WA, Bertrand E, Coulombe B.

The PAQosome, an R2TP-Based Chaperone for Quaternary Structure Formation

Trends Biochem Sci. 2018 Jan;43(1):4-9. doi: 10.1016/j.tibs.2017.11.001.

Schematic of the PAQosome Structure, Adaptors, and Clients

Discovery of leukodystrophy-causing mutations that target assembly of RNA pol III

Thiffault I, Wolf NI, Forget D, Guerrero K, Tran LT, Choquet K, Lavallée-Adam M, Poitras C, Brais B, Yoon G, Sztriha L, Webster RI, Timmann D, van de Warrenburg BP, Seeger J, Zimmermann A, Máté A, Goizet C, Fung E, van der Knaap MS, Fribourg S, Vanderver A, Simons C, Taft RJ, Yates JR 3rd, Coulombe B, Bernard G.

Recessive mutations in POLR1C cause a leukodystrophy by impairing biogenesis of RNA polymerase III

Nat Commun. 2015 Jul 7;6:7623. doi: 10.1038/ncomms8623.